The second European Joint Action on Antimicrobial Resistance and Healthcare-Associated Infections (EU-JAMRAI2) is dedicated to a multifaceted approach aimed at combating antimicrobial resistance (AMR) across Europe. Our objectives encompass raising awareness and understanding of AMR throughout the continent, ensuring that individuals and communities are informed and empowered to address this critical issue. Moreover, we strive to provide continuity and encourage the wider adoption of policy recommendations established during EU-JAMRAI 1, fostering a cohesive and sustainable response to AMR. By promoting best practices and facilitating knowledge-sharing, we aim to make Europe a model region in the fight against AMR. Additionally, our efforts extend to raising awareness of the importance of prudent antimicrobial consumption in the human and animal health, and in the environment, underscoring the interconnectedness of these domains in mitigating the threat of AMR and safeguarding public health.